Resilient microorganisms in dust samples of the International Space Station—survival of the adaptation specialists

نویسندگان

  • Maximilian Mora
  • Alexandra Perras
  • Tatiana A. Alekhova
  • Lisa Wink
  • Robert Krause
  • Alina Aleksandrova
  • Tatiana Novozhilova
  • Christine Moissl-Eichinger
چکیده

BACKGROUND The International Space Station (ISS) represents a unique biotope for the human crew but also for introduced microorganisms. Microbes experience selective pressures such as microgravity, desiccation, poor nutrient-availability due to cleaning, and an increased radiation level. We hypothesized that the microbial community inside the ISS is modified by adapting to these stresses. For this reason, we analyzed 8-12 years old dust samples from Russian ISS modules with major focus on the long-time surviving portion of the microbial community. We consequently assessed the cultivable microbiota of these samples in order to analyze their extremotolerant potential against desiccation, heat-shock, and clinically relevant antibiotics. In addition, we studied the bacterial and archaeal communities from the stored Russian dust samples via molecular methods (next-generation sequencing, NGS) and compared our new data with previously derived information from the US American ISS dust microbiome. RESULTS We cultivated and identified in total 85 bacterial, non-pathogenic isolates (17 different species) and 1 fungal isolate from the 8-12 year old dust samples collected in the Russian segment of the ISS. Most of these isolates exhibited robust resistance against heat-shock and clinically relevant antibiotics. Microbial 16S rRNA gene and archaeal 16S rRNA gene targeting Next Generation Sequencing showed signatures of human-associated microorganisms (Corynebacterium, Staphylococcus, Coprococcus etc.), but also specifically adapted extremotolerant microorganisms. Besides bacteria, the detection of archaeal signatures in higher abundance was striking. CONCLUSIONS Our findings reveal (i) the occurrence of living, hardy microorganisms in archived Russian ISS dust samples, (ii) a profound resistance capacity of ISS microorganisms against environmental stresses, and (iii) the presence of archaeal signatures on board. In addition, we found indications that the microbial community in the Russian segment dust samples was different to recently reported US American ISS microbiota.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

بررسی سطح گرد وغبار و سیلیس آزاد موجود درهوای معادن سنگ آهن خواف

Background and Objectives: Silica is an inorganic material that found in the nature in various forms. The hazards of crystal of silica or α-quartz (free silica) is greater than other forms. Exposure to dust that contains free silica result to pulmonary injury and result to silicosis and death finally. The present research was conducted in order to evaluate the level of workers exposure to free ...

متن کامل

The Temporal Analysis of Dust Storms in Khoramabad Synoptic Station

In this study, the changes in the Khorramabad storm in the period of 1952 to 2015 have been investigated. For this purpose, data from meteorological codes 06 and 07 were received from the Meteorological Organization of the country, and after identifying the days of winding with dust storms and calculating their monthly frequency, monthly, seasonal and annual time series were analyzed. In this s...

متن کامل

Investigation of dust and evaluation of its prediction in Ardebil province using ANFIS model

Investigation and evaluation of dust and microstrip phenomena is one of the important values ​​in the management of climate and environmental hazards in the Middle East, especially in the arid, western, southern and central parts of Iran. Methods and plans for studying this phenomenon and its management are of great importance and great value. According to studies on dust phenomena based on pre...

متن کامل

Assessment of impact of climate change on potato and potential adaptation gains in the Indo-Gangetic Plains of India

India is the second largest producer of potato in the world. The Indo-Gangeticplains (IGP) is the main potato growing region accounting for almost 85% of the1.8 Mha under the crop in India where it is grown as an irrigated crop during thewinter season. Since IGP is in sub-tropical plains, duration of the thermally suitablewindow is the main determinant limiting yields. Hence the impact of clima...

متن کامل

Selenuim-enriched probiotic fermented milk drink (DOOGH): characteristics and survival microorganisms

Selenium (Se) is considered as an essential element for human body. Selenized probiotic fermented milk drink (DOOGH) as a functional beverage to increase Se available in body. The predominate sugar, protein and mineral ion of Iranian yoghurt drink (DOOGH) are lactose, casein and calcium, respectively. Different levels of  Selenium (2.5 – 3- 3,5-  4 and 4.5) is prepared and its suitability based...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 4  شماره 

صفحات  -

تاریخ انتشار 2016